Phenotypic and genotypic characterization of peptidoglycan hydrolases of Lactobacillus sakei

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Phenotypic and genotypic characterization of peptidoglycan hydrolases of Lactobacillus sakei

Lactobacillus sakei, a lactic acid bacterium naturally found in fresh meat and sea products, is considered to be one of the most important bacterial species involved in meat fermentation and bio-preservation. Several enzymes of Lb. sakei species contributing to microbial safeguarding and organoleptic properties of fermented-meat were studied. However, the specific autolytic mechanisms and assoc...

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Integrated phenotypic-genotypic approach to understand the influence of ultrasound on metabolic response of Lactobacillus sakei

The lethal effects of soundwaves on a range of microorganisms have been known for almost a century whereas, the use of ultrasound to promote or control their activity is much more recent. Moreover, the fundamental molecular mechanism influencing the behaviour of microorganisms subjected to ultrasonic waves is not well established. In this study, we investigated the influence of ultrasonic frequ...

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Phenotypic and Genotypic Characterization of Indigenous Lactobacillus Species from

Lactobacilli are well reckoned for their technological and probiotic significance and their exercise in dairy products for trade and industry. The present study was carried out to isolate and characterize Lactobacilli from various dairy and non dairy sources of Indian origin. The isolates were subjected to phenotypic and genotypic characterizations followed by partial 16S rDNAsequencing . As ma...

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Identification of key peptidoglycan hydrolases for morphogenesis, autolysis, and peptidoglycan composition of Lactobacillus plantarum WCFS1

BACKGROUND Lactobacillus plantarum is commonly used in industrial fermentation processes. Selected strains are also marketed as probiotics for their health beneficial effects. Although the functional role of peptidoglycan-degrading enzymes is increasingly documented to be important for a range of bacterial processes and host-microbe interactions, little is known about their functional roles in ...

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Purification and characterization of an arginine aminopeptidase from Lactobacillus sakei.

An arginine aminopeptidase (EC 3.4.11.6) that exclusively hydrolyzes basic amino acids from the amino (N) termini of peptide substrates has been purified from Lactobacillus sakei. The purification procedure consisted of ammonium sulfate fractionation and three chromatographic steps, which included hydrophobic interaction, gel filtration, and anion-exchange chromatography. This procedure resulte...

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ژورنال

عنوان ژورنال: Journal of Advanced Research

سال: 2016

ISSN: 2090-1232

DOI: 10.1016/j.jare.2015.04.004